Development and validation of a novel reporter assay for human papillomavirus type 16 late gene expression
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چکیده
منابع مشابه
Development and validation of a novel reporter assay for human papillomavirus type 16 late gene expression.
To facilitate the investigations of HPV-16 late gene expression HPV-16 reporter plasmids were generated using previously described sub-genomic HPV-16 plasmids, named pBEL and pBELM, that, similar to the full viral genome, produce primarily HPV-16 early mRNAs and very little, if any, late mRNAs in cervical cancer cells. The HPV-16 late L1 gene was replaced by the chloramphenicol acetyltransferas...
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متن کاملdeveloping michigan cancer foundation 7 cells with stable expression of e7 gene of human papillomavirus type 16
background: human papillomavirus (hpv) is responsible for the development of cervical neoplasia. infection with human papillomavirus type 16 (hpv-16) is a major risk factor for the development of cervical cancer. the virus encodes three oncoproteins (e5, e6 and e7), of which, the e7 oncoprotein is the major protein involved in cell immortalization and transformation of the infected cells. the ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2012
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2012.03.023